Invitrogen™ Apolipoprotein J Polyclonal Antibody

Product Code.	Quantity	unitSize
16384545	100 μg	100µg

Product Code. 16384545 Supplier Invitrogen™ Supplier No. PA595280

Description

Rabbit Polyclonal Antibody

Human Apolipoprotein J shares 76.1 % and 75.2 % amino acid (aa) sequence identity with mouse and rat Apolipoprotein J, respectively. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: SKOV whole cell, U87 whole cell, PANC whole cell. IHC: human testis tissue. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Specifications

• Antigen: Apolipoprotein J
• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide
• Gene: Clu
• Gene Accession No.: P10909
• Gene Alias: AAG4; Aging associated protein 4; Aging-associated gene 4 protein; aging-associated protein 4; AI893575; apo j; Apo J a; Apo J alpha; Apo J b; Apo J beta; Apo J α; Apo J β; Apo Ja; Apo Jb; ApoJ; Apo-J; ApoJ a; ApoJ alpha; ApoJ b; ApoJ beta; ApoJ α; ApoJ β; ApoJa; ApoJalpha; ApoJb; ApoJbeta; Apolipoprotein J; CLI; Clu; CLU1; CLU2; clusterin; Clusterin alpha / beta chain; Clusterin alpha chain; Clusterin beta chain; Clustrin; Complement associated protein SP40; complement cytolysis inhibitor; Complement cytolysis inhibitor a chain; Complement cytolysis inhibitor b chain; complement lysis inhibitor; complement-associated protein SP-40,40; D14Ucla3; DAG; Dimeric acid glycoprotein; GP80; HGNC:2095; ku70-binding protein 1; Ku70-binding protein 1 (KUB1); KUB1; MGC24903; Msgp-2; NA1/NA2; RATTRPM2B; SGP2; Sgp-2; SP40; SP-40; Sugp-2; sulfated glycoprotein 2; Sulfated glycoprotein 2 (SGP2); testosterone repressed prostate message; testosterone repressed prostate message 2; Testosterone repressed prostate message 2 (TPRM2B); testosterone repressed prostate message-2; testosterone-repressed prostate message; testosterone-repressed prostate message 2; testostrone-repressed prostate message 2; Trpm2; TRPM-2; TRPM2B; Trpmb
• Gene Symbols: Clu
• Host Species: Rabbit
• Immunogen: E.coli-derived human Apolipoprotein J recombinant protein (Position: S228-E449).
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1191
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG