APIP Monoclonal Antibody (19F461), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA141247

Product Code. 11569822

Description

Suggested positive control: HeLa or 293, antigen standard for APIP (transient overexpression lysate).

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf 1 (for apoptosis protease-activating factor 1). Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), which leads to Caspase-9 activation. Activated Caspase-9 in turn cleaves and activates Caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Recently, Cho et al (4) have identified a new Apaf 1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf 1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Specifications

• Antigen: APIP
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.05% BSA and 0.05% sodium azide
• Gene Accession No.: Q96GX9, Q9WVQ5
• Gene Symbols: APIP
• Immunogen: Recombinant APIP protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: 19F461
• Conjugate: Unconjugated
• Gene: APIP
• Gene Alias: APAF1 interacting protein; APAF1-interacting protein; APIP; APIP2; CG129; CGI29; CGI-29; dJ179L10.2; hAPIP; Methylthioribulose-1-phosphate dehydratase; Mmrp19; monocyte macrophage 19; monocyte/macrophage protein 19; MTRu-1-P dehydratase; probable methylthioribulose-1-phosphate dehydratase; RGD1564562
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 51074, 56369
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid