Invitrogen™ APE1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA116500

Product Code. 11535293

Description

In IHC applications, this antibody can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. This antibody can be used on frozen sections, fixed-paraffin sections and cytospin preps. Suggested positive control: Hela whole cell extract, antigen standard for APEX1 (transient overexpression lysate).

Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.

Specifications

• Antigen: APE1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: APEX1
• Gene Alias: AP endonuclease 1; AP endonuclease class I; AP lyase; Ape; APE1; APEN; Apex; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apex1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endodeoxyribonuclease 1; apurinic/apyrimidinic endonuclease; apurinic/apyrimidinic endonuclease 1; Apurinic-apyrimidinic endonuclease 1; APX; BAP 1; BAP1; deoxyribonuclease (apurinic or apyrimidinic); DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase, mitochondrial; HAP1; protein REF-1; redox factor 1; redox factor-1; REF1; REF-1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100348924, 11792, 328, 79116
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ChIP Assay, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: P27695, P28352, P43138
• Gene Symbols: APEX1
• Immunogen: Affinity purified human APE1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Rabbit, Monkey
• Product Type: Antibody
• Isotype: IgG