APAF1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA519894

Product Code. 12828112

Description

In Western blot applications, this antibody detects a band at ∽130kDa. A suggested positive control is human heart tissue lysate.

APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Specifications

• Antigen: APAF1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: APAF1
• Gene Alias: 6230400I06Rik; APAF1; APAF-1; Apaf1l; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; apoptotic protease-activating factor 1; CED4; DKFZp781B1145; fog; forebrain overgrowth; KIAA0413; mKIAA0413
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11783, 317, 78963
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: O14727, O88879, Q9EPV5
• Gene Symbols: APAF1
• Immunogen: A peptide corresponding to amino acids near the carboxy terminus of human Apaf1
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG