V5 Tag, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen R96025
216.90 GBP valid until 2019-02-28
Use promo code "13474" to get your promotional price.
Code : 50
Additional Details : Weight : 0.00525kg
DescriptionNOTE: R960-25 was previously sold under product #46-0705. When re-ordering, please use #R960-25.R960-25 recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.This antibody is functionally tested against 20ng of an E. coli expressed fusion protein containing a V5 epitope using a chemiluminescent substrate at a 1 minute exposure. This antibody has also been tested in Western blot against 25ng of recombinant Positope™ protein. The Positope™ control protein is a 53kDa recombinant protein that contains seven epitope tags, including His (C-term), HisG, c-myc, and V5. Low background was observed using chemiluminescent or alkaline phosphatase reagents for detection. For Western blot, dilute in PBS or Tris-Buffered Saline (TBS) containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM or TBSTM).Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.This antibody has also been used successfully to immunoprecipitate fusion proteins that contain theV5 epitope.
|PBS with 0.01% sodium azide|
|V5 Epitope Tag|
|V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.|
|ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot, Western Blot|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles., Store at -20°C, avoiding freeze/thaw cycles.|