Thrombin Receptor/PAR1 Mouse, Unlabeled, Clone: 14, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 611523

Product Code. 15895959

Description

Thrombin is a serine protease that regulates the activity of many cell types including the activation of platelets after vascular injury. Thrombin functions are mediated by G-protein-coupled protease-activated receptors that are homologous to substance P and thyrotropin receptors. Thrombin receptors (PAR1, PAR3, and PAR4) are seven transmembrane domain proteins with large N-terminal exodomains that contain a thrombin cleavage site (LDPR/S). Thrombin binds to the exodomain and cleaves the peptide bond between Arg-41 and Ser-42. This unmasks a new N-terminus with the sequence SFLLRN that acts as a tethered ligand. SFLLRN binds to the body of the thrombin receptor leading to irreversable activation. Both phosphorylation of the thrombin receptor and internalization may uncouple downstream signaling. PAR1 protein is expressed in vascular endothelial cells, smooth muscle cells, and macrophages, while PAR1 mRNA is expressed at higher levels in neonatal rat brain and lower levels in skeletal muscle, liver, and kidney. Thus, G-protein signaling via thrombin receptors, like PAR1, may be important for a diverse array of cellular functions, such as platelet activation and neural development.

Host Species: Mouse
Clone: 14
Isotype: IgG1
Species Reactivity: Human
Immunogen: Human Thrombin Receptor/PAR1 aa. 33-153
Formula Weight [Chemical]: 47kDa

Immunofluorescence, Western Blotting

Specifications

• Antigen: Thrombin Receptor/PAR1
• Classification: Monoclonal
• Concentration: 250μg/mL
• Description: Protease Activated Receptor-1 (PAR1)
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

• Applications: Western Blot
• Clone: 14
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Human Thrombin Receptor/PAR1 aa. 33-153
• Quantity: 150 μg
• Research Discipline: Cell Biology
• Target Species: Human, Mouse
• Isotype: IgG1