TGF beta-1 Mouse anti-Mouse, Clone: 9016, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA523702

Product Code. 15553392

Description

In sandwich ELISAs, less than 2% cross-reactivity with recombit human (rh) TGF- beta 3 and recombit amphibian TGF- beta 5 and no cross-reactivity with recombit porcine TGF-beta 2 or rhTGF-beta 2 is observed. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is <0.10 EU per 1 μg of the antibody by the LAL method.

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.

Specifications

• Antigen: TGF beta-1
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: P04202
• Gene Symbols: TGFB1
• Immunogen: CHO-derived recombit TGF-beta 1 and latent TGF-beta 1
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Neutralization, Western Blot
• Clone: 9016
• Conjugate: Unconjugated
• Gene: Tgfb1
• Gene Alias: TGFB, TGFb-1, DPD1, Transforming Growth Factor, Transforming Growth Factor Beta-1, TGF-beta-1, CED
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 21803
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized