TBK1 (pS172) Mouse, Unlabeled, Clone: J133-1171, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 558397

Product Code. 15859348

Description

NFκB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. In most cells, NF-κB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IkBα, IkBβ, and IkBε. Stimulation induces the release, activation, and nuclear translocation of NF-κB. Release of NF-κB results from the phosphorylation and proteolytic degradation of the IκB proteins. Two cytokine-inducible IκB kinases (IKKα and IKKβ) phosphorylate and target the IκB proteins for degradation via the ubiquitin pathway. IKKγ/NEMO, a third member of the IKK complex, functions as a regulatory subunit and interacts directly with IKKb. TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein of 729 amino acids, is another member of the IKK family of kinases regulating NF-κB downstream of the tumor necrosis factor and Toll-like receptor pathways. TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-κB activator) and TRAF2 (TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172 (S172). The J133-1171 monoclonal antibody recognizes the phosphorylated S172 of human TBK1.

Western Blotting

Specifications

• Antigen: TBK1 (pS172)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: T2K, NAK
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Western Blot
• Clone: J133-1171
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human TBK1
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human, Mouse
• Isotype: IgG2b κ