TCR V beta 3 Mouse anti-Human, Clone: JOVI.1, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA190957

Product Code. 11581052

Description

MA1-90957 detects T Cell receptor from human samples. MA1-90957 has been successfully used in flow cytometry, immunohistochemistry (frozen tissue), immunoprecipitation applications. The MA1-90957 immunogen is: Thymus, spleen and mesenteric lymph nodes isolated from a mouse transgenic for human Vb3 TcR. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

Specifications

• Antigen: TCR V beta 3
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Gene Alias: TCRVB3; TCRV beta3; TCR Vb3
• Immunogen: Thymus, spleen and mesenteric lymph nodes isolated from a mouse transgenic for human Vb3 TcR.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: JOVI.1
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a