Invitrogen™ Shiga like-toxin 1 Monoclonal Antibody (13C4)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA140277

Product Code. 11569752

Description

MA1-40277 detects Shigatoxin 1 from E. coli samples. MA1-40277 has been successfully used in ELISA, functional assays and Western blot applications. The MA1-40277 immunogen is 1B subunit of Shigatoxin 1.

Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share 99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.

Specifications

• Antigen: Shiga like-toxin 1
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.1% BSA and no preservative
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Functional Assay, Western Blot
• Clone: 13C4
• Conjugate: Unconjugated
• Gene Alias: SLT1; SLT-1; SLTI; SLT-I; Verocytotoxin; Verotoxin
• Immunogen: 1B subunit of Shigatoxin 1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid