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Invitrogen™ S100A1 Polyclonal Antibody
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Product Code. 11568611
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Quantity:
100 μg
Unit Size:
100µg
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11568611 100 μg 100µg
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Product Code. 11568611 Supplier Invitrogen™ Supplier No. PA1932

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Description

Rabbit Polyclonal Antibody

PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein. PA1-932 has successfully been used in Western blot, ELISA, immunofluorescence, immunocytochemistry, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932. S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane. The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells. Reconstitute in 100 μL PBS to create a stock of 1 mg/mL.

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
TRUSTED_SUSTAINABILITY

Specifications

Antigen S100A1
Applications ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 20mg/mL BSA and 0.1% sodium azide
Gene S100A1
Gene Accession No. P02639, P23297, P35467, P56565
Gene Alias AI266795; Protein S100-A1; S100; S100 alpha; S100 calcium binding protein A1; S100 calcium-binding protein A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 protein, alpha polypeptide; S100a; S100A1; S100-alpha
Gene Symbols S100A1
Host Species Rabbit
Immunogen Purified S100-alpha protein from human pectoral muscle cells.
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 106504218, 20193, 295214, 528735, 6271
Target Species Human, Mouse, Rat, Bovine, Pig
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Lyophilized
Isotype IgG
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