ROCK-II Mouse, Unlabeled, Clone: 21, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 610624

Product Code. 15865549

Description

Activity of the GTP-binding proteins is regulated by GAPs, that accelerate binding, and GTPases, that enhance the rate of GTP hydrolysis (Ras, Rho, cdc42Hs, and Rac). The isoforms of Rho, a small GTP-binding protein, regulate cellular processes such as the formation of the stress fibers, lamelipodia, and filopodia. ROKα (RhoA-binding kinase) is a Ser/Thr protein kinase that interects with the GTP-binding form of RhoA. Like RhoA, ROKα localizes at the cellular margins and colocalizes with acti filaments. The ROKα gene encodes a protein of 1302 amino acids with homology to the human myotonic kinase. Although ROKα binds to GTP-RhoA, it is not yet clear whether this interaction induces the kinase activity of ROKα.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: ROCK-II
• Classification: Monoclonal
• Concentration: 250μg/mL
• Description: ROKα
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

• Applications: Western Blot
• Clone: 21
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Rat ROKα aa. 567-718
• Quantity: 150 μg
• Research Discipline: Cell Biology
• Target Species: Canine, Human, Mouse, Rat
• Isotype: IgG1

For Research Use Only.