RACK1 Mouse, Unlabeled, Clone: 20, BD

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15825309	BD Biosciences 610177	50 μg
15835309	BD Biosciences 610178	150 μg

Description

Several proteins which specifically bind to PKC have been classified as RACKs (receptors for activated C-kinase). RACK1 was cloned from a rat brain cDNA expression library by screening for proteins that bind PKC in the presence of phosphatidylserine, diacylglycerol, and calcium in a PKC overlay assay. By sequence homology, RACK1 appears to belong to a superfamily that includes the β subunit of G proteins. All of these proteins contain five to eight internal repeat elements known as WD40 motifs, which appear to have a role in protein-protein interactions. In addition, RACK1 contains two short sequences homologous to a PKC-binding sequence identified in Annexin I and in the brain PKC inhibitor KCIP. The binding of RACK1 to PKC is dose-dependent and occurs at a site on PKC that is distinct from the catalytic domain, indicating that RACK1 is not a PKC substrate.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: RACK1
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgM
• Purification Method: Affinity Purified

• Clone: 20
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Bovine, Canine, Chicken, Frog, Human, Mouse, Rat
• Immunogen: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.