Invitrogen™ Phospho-(Ser/Thr) ATM + ATR Substrate Monoclonal Antibody (G.770.10)

Rabbit Monoclonal Antibody

Brand:  Invitrogen™ MA514872

Product Code. 11382062

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. This antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position.

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation. The complex phenotype of AT cells suggests that it likely has additional substrates.

Specifications

• Antigen: Phospho-(Ser/Thr) ATM + ATR Substrate
• Classification: Monoclonal
• Concentration: 192 μg/mL
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: All species
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: G.770.10
• Conjugate: Unconjugated
• Gene Alias: L(s/t)Q; SQ/TQ
• Immunogen: Synthetic phospho-ATM/ATR substrate peptides.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: -20°C
• Form: Liquid