Perforin Mouse anti-Human, Unlabeled, Clone: ΔG9, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 556434

Product Code. 15818698

Description

Perforin has a key role in cell-mediated cytotoxicity. It is a 70kDa cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses, in allograft rejections, and in some autoimmune diseases. NK cells are important for tumor surveillance and destruction and are involved in allograft rejections. Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell. In the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that resembles apoptosis. The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding perforin-mediated reactions. Clone δG9 reacts with human and bovine perforin. It does not cross-react with mouse perforin. Purified granules from the human lymphoma cell line YT were used as immunogen. Clone δG9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen tissue sections. The antibody stains scattered lymphocytes in red pulp of spleen, and scattered infiltrated lymphocytes in lymphoma.

Host Species: Mouse
Clone: δG9
Isotype: IgG2b, κ
Species Reactivity: Human
Immunogen: Purified Granules from the Human Lymphoma Cell Line YT

Flow Cytometry, Immunofluorescence, Immunohistochemistry (Frozen), Immunoprecipitation

Specifications

• Antigen: Perforin
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: PRF1; P1; PERF; PFN1; PFP; Perforin-1; Cytolysin; FLH2; HPLH2
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Flow Cytometry
• Clone: ΔG9
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Purified Granules from the Human Lymphoma Cell Line YT
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Bovine, Human
• Isotype: IgG2b κ

For Research Use Only.