Invitrogen™ p27 Kip1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA527188

Product Code. 13491297

Description

Recommended positive controls: HeLa, MCF-7, MDA-MB-231, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12. Predicted reactivity: Mouse (80%), Dog (87%), Cat (87%), Pig (84%), Rhesus Monkey (98%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Specifications

• Antigen: p27 Kip1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Cdkn1b
• Gene Alias: AA408329; AI843786; Cdki1b; CDKN1B; CDKN4; CDN1B; cyclin dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B (p27 Kip1); Cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; cyclin-dependent kinase inhibitor p27/Kip1; KIP1; MEN1B; MEN4; p27; P27 CDKN1B; p27(KIP1); p27Kip1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1027, 12576
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.07 mg/mL
• Formulation: PBS with 20% glycerol, 1% BSA and 0.025% ProClin 300; pH 7
• Gene Accession No.: P46414, P46527
• Gene Symbols: Cdkn1b
• Immunogen: Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG