NR4A1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA527274

Product Code. 13421467

Description

Recommended positive controls: HepG2, HepG2 (treatment 1% Hypoxia for 24 hr), NUR77 tansfected 293T cell, mouse liver. Predicted reactivity: Mouse (92%), Rat (94%), Dog (96%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.

Specifications

• Antigen: NR4A1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NR4A1
• Gene Alias: early response protein NAK1; Gfrp; GFRP1; growth factor-inducible nuclear protein N10; Hbr1; Hbr-1; Hmr; hormone receptor; immediate early gene transcription factor NGFI-B; MGC9485; N10; NAK1; NAK-1; nerve growth factor IB nuclear receptor variant 1; nerve growth factor induced protein I-B; nerve growth factor-induced protein I-B; Ngfib; NGFI-B; NP10; Nr4a1; Nuclear hormone receptor NUR/77; nuclear protein N10; Nuclear receptor subfamily 4 group A member 1; nuclear receptor subfamily 4, group A, member 1; NUR77; Orphan nuclear receptor HMR; orphan nuclear receptor TR3; Orphan receptor tr3; ST-59; steroid receptor TR3; testicular receptor 3; TIS1; TR3; TR3 orphan receptor
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15370, 3164
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Western Blot
• Concentration: 0.23 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P12813, P22736
• Gene Symbols: NR4A1
• Immunogen: Recombinant fragment corresponding to a region within amino acids 361 and 589 of Human NUR77
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG