Nur77 Mouse anti-Mouse, Unlabeled, Clone: 12.14, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 554088

Product Code. 15837568

Description

Nur77 (also known as NGF1-B, N10, TISI) is a growth factor-inducible orphan member of the steroid/thyroid hormone receptor superfamily. This superfamily encodes ligand-dependent transcription factors with a centrally located, highly conserved DNA-binding domain containing two zinc-fingers. Although Nur77 binds no known ligand, it is constitutively active when synthesized. Nur77 was originally identified as an immediate-early gene rapidly activated by serum stimulation of quiescent fibroblasts. It has since been shown to be activated by diverse signals including membrane depolarization, nerve growth factor, chemically induced seizures, adrenocorticotrophic hormone (ACTH), pentylene tetrazole, forskolin and cAMP. Nur77, like other immediate-early genes such as c-myc, has also been shown to have a role in apoptosis. Apoptosis is an internal, programmed cell death which takes place during normal development. Nur77 has been demonstrated to be required for in vitro T-cell-receptor (TCR) mediated negative selection. Negative selection, or the clonal deletion of thymocytes, normally occurs by apoptosis following engagement of the TCR. Nur77 is present in high levels in T-cell hybrids and thymocytes undergoing apoptosis, but not in growing T cells or stimulated splenocytes. T-cell hybrids are protected from activation-induced apoptosis by a Nur77 dominant negative mutation. Induction of Nur77 mRNA and cell death by apoptosis following treatment of T-cell hybrids with antibody directed against the TCR has also been shown. Additionally, transfection of the T-cell hybrids with antisense Nur77 protects cells from apoptosis when signaled to die by TCR engagement. Nur77 is a phosphoprotein which migrates on SDS/PAGE gels as diffuse bands between 67 and 88kDa depending on post-translational modifications. Clone 12.14 recognizes mouse Nur77. A full-length mouse Nur77 fusion protein was used as immunogen.

Host Species: Mouse
Clone: 12.14
Isotype: IgG1
Species Reactivity: Mouse
Immunogen: Full-length mouse Nur77 fusion protein
Formula Weight [Chemical]: 67-88kDa

Western Blotting

Specifications

• Antigen: Nur77
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: Nr4a1; NGFIB; NGFI-B; GFRP1; Gfrp; N10; NP10; TR3 ; Hbr1; Hbr-1; Hmr; TIS1
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Western Blot
• Clone: 12.14
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Full-length mouse Nur77 fusion protein
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Mouse
• Isotype: IgG1

For Research Use Only.