NBS1 Mouse, Unlabeled, Clone: 34, BD

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15836049	BD Biosciences 611871	150 μg

Description

Nijmegen Breakage Syndrome (NBS) is characterized by extreme radiation sensitivity and chromosomal instability. The NBS1 gene product, p95/nibrin/NBS1, forms a complex with Rad50 and Mre11. Cells deficient in this complex have problems with DNA double-strand break repair, cell cycle checkpoint control, and telomere length maintenance. NBS1 contains a forkhead-associated domain (FHA) and a breast cancer carboxy-terminal domain (BRCT) in the N-terminal region. Both of these domains have been found in DNA-damage responsive cell cycle checkpoint proteins. The complex containing NBS1, Rad50, and Mre11 possesses manganese-dependent single stranded DNA endonuclease and 3′ to 5′ exonuclease activities. In addition, NBS1 is required for DNA-damage dependent phosphorylation of Mre11. This phosphorylation may be required for proper nuclear localization of the NBS1-Rad50-Mre11 complex to sites of DNA double-strand breaks. NBS1 interacts directly with telomere repeat binding factor, TRF1, via its C-terminal region, and both NBS1 and Mre11 co-localize with TRF1 at promyelocytic leukemia nuclear bodies. Thus, the NBS1-Rad50-Mre11 complex may be important for both DNA damage repair, and telomere length maintenance.

Host Species: Mouse
Clone: 34
Isotype: IgG1
Species Reactivity [for Features Main]: Human
Immunogen: Human NBS1 aa. 620-732

Immunofluorescence, Western Blotting

Specifications

• Antigen: NBS1
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgG1
• Purification Method: Affinity Purified
• Description: Nijmegen Breakage Syndrome-1

• Clone: 34
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Canine, Chicken, Human, Mouse, Rat
• Immunogen: Human NBS1 aa. 620-732
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.