SCN5A Monoclonal Antibody (4G8), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA127429

Product Code. 11519602

Description

MA1-27429 detects Nav 1.5 from human samples. MA1-27429 may be successfully used in immunocytochemistry, immunofluorescence and Western blot applications. The MA1-27429 immunogen is a synthetic peptide corresponding to an extracellular domain of Nav1.5.

Scn5a is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. Scn5a is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in Scn5a are a cause of long QT syndrome type 3 (LQT3), an autosomal domit cardiac disease. Alternative splicing results in several transcript variants encoding different isoforms of Scn5a. Voltage-gated sodium channesl (VGSCs) mediate regenerative cell membrane depolarization and conduction of electrical signalling in nerves and muscles. Expression of Scn5a is also detected in lymphocytes, glia, and fibroblasts. Further, Scn5a mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, Scn5a forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. Functionally, Scn5a is responsible for the initial upstroke of the action potential and the channel inactivation is regulated by intracellular calcium levels. Voltage-gated sodium channels (NaV) are responsible for action potential initiation and propagation in excitable cells, including nerve, muscle, and neuroendocrine cell types. Scn5a are also expressed at low levels in non-excitable cells, where their physiological role is unclear. Diseases associated with SCN5A include Long Qt Syndrome-3 and Brugada Syndrome 1.

Specifications

• Antigen: SCN5A
• Classification: Monoclonal
• Concentration: 0.9 mg/mL
• Gene: SCN5A
• Gene Symbols: SCN5A
• Immunogen: Synthetic peptide corresponding to a portion of the extracellular domain of Nav1.5.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgM

• Applications: Immunocytochemistry, Immunofluorescence, Western Blot
• Clone: 4G8
• Conjugate: Unconjugated
• Gene Accession No.: Q14524
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6331
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid