MDM2 Mouse anti-Human, Clone: 2A10, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA125896

Product Code. 11529402

Description

MA1-25896 detects MDM2 from human samples. MA1-25896 has been successfully used in immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and Western blot procedures. Heat pretreatment is required for paraffin sections. The MA1-25896 immunogen is human MDM2 protein.

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human maligcies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.

Specifications

• Antigen: MDM2
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Gene: MDM2
• Gene Alias: HDMX, MGC5370, MGC71221, hdm2
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 4193
• Content And Storage: 4° C, do not freeze
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: 2A10
• Conjugate: Unconjugated
• Gene Accession No.: Q00987
• Gene Symbols: MDM2
• Immunogen: Human MDM2 protein.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a