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Description
Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation
results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38kDa forms that result from post-translational modification, and as a 42kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCγ1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development. The I58-1169 monoclonal antibody recognizes the phosphorylated tyrosine 171 (pY171) of LAT, which is one of the phosphotyrosine sites required for binding phosphoinositide 3-kinase, Grb2, and Gads.
Immunohistochemistry, Western Blotting
Specifications
| LAT (pY171) | |
| Monoclonal | |
| 0.5mg/mL | |
| Aqueous buffered solution containing ≤0.09% sodium azide. | |
| Phosphorylated peptide of the region including the tyrosine 171 of the short isoform of human LAT | |
| 0.1 mg | |
| Cell Biology | |
| Human | |
| IgG1 κ |
| Western Blot | |
| I58-1169 | |
| Unconjugated | |
| Mouse | |
| Affinity Purified | |
| RUO | |
| Primary | |
| Store undiluted at 4°C |
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