IRAK2 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA520163

Product Code. 12878322

Description

This antibody reacts with all four isoforms or IRAK2a. In Western blot applications, this antibody detects a band at ∽65kDa. A suggested positive control is RAW264.7 cell lysate. PA5-20163 can be used with blocking peptide PEP-0280.

IRAK2 (interleukin-1 receptor-associated kinase-like 2) binds to the IL-1 type I receptor following IL-1 engagment and triggers intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappa-B, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappa-B activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappa-B activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory responses to cytokine IL-1 and LPS. IRAK2 is expressed in a variety of human tissues.

Specifications

• Antigen: IRAK2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: IRAK2
• Gene Alias: 6330415L08Rik; AI649099; interleukin 1 receptor associated kinase 2; interleukin-1 receptor associated kinase-2; interleukin-1 receptor-associated kinase 2; interleukin-1 receptor-associated kinase 2c; interleukin-1 receptor-associated kinase-like 2; Irak2; IRAK-2; MGC150550; mu-IRAK-2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 108960, 3656
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunocytochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: O43187, Q8CFA1
• Gene Symbols: IRAK2
• Immunogen: A peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG