Granzyme A Set Mouse anti-Human, FITC, Clone: CB9, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 558905

Product Code. 15829648

Description

The primary mechanism by which cytotoxic T cells eliminate virally infected cells is by granule exocytosis. The release of cytotoxic granule contents by cytotoxic T lymphocytes (CTL) triggers apoptotic target cell death. CTL granules contain a pore-forming protein, perforin, and a group of serine proteases called granzymes. In the classic model, perforins create holes in the target cell membrane, allowing entrance of the granzymes. Granzyme A and B are the predominant granzymes activated after CTL activation, but each act via an independent apoptotic pathway; granzyme B is activated immediately, while granzyme A acts hours later. Granzyme B does not induce cleavage of caspase-3, lamin B, rho-GTPase or PARP, but does cleave DNA-PKcs and nuclear mitotic apparatus protein (NuMA). The physiological substrates for granzyme A in the apoptotic pathway have not been identified. Studies involving mice which are deficient in both granzyme A and B suggest a model whereby the granzyme B pathway may have evolved as the major apoptotic pathway with the granzyme A pathway acting as a backup. However, further research is needed to delineate the components of the distinct pathways.

Clone CB9 recognizes human granzyme A. Purified human granzyme A was used as immunogen. Clone MOPC-21 is a mouse IgG1 isotype (negative) control. The MOPC-21 antibody has unknown specificity. CB9 and MOPC-21 FITC conjugates are optimized in F/P ratios.

The antibodies are routinely tested in parallel by flow cytometry. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: Granzyme A Set
• Classification: Monoclonal
• Conjugate: FITC
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Includes: FITC Human Granzyme A (100 tests), FITC Mouse IgG1, κ Isotype Control (100 tests)
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: CB9
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG1