Granulocytes Mouse anti-Rat, PE, Clone: RP-1, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 550002

Product Code. 15896158

Description

The RP-1 antibody reacts with rat peritoneal and peripheral blood neutrophils and bone marrow cells. It does not bind rat macrophages, rat eosinophils, or peritoneal neutrophils from mice, rabbits, guinea pigs, or human peripheral blood as determined by antibody adsorption experiments. Flow cytometric analysis of peripheral blood leukocytes indicates that all granulocytes (cells having high side scatter), but not lymphocytes or monocytes (low side-scatter cells), express that antigen recognized by RP-1 mAb. Expression of the RP-1 antigen on rat
peritoneal neutrophils is enhanced by stimulation with PMA or ConA. Immunoprecipitation of rat PMA-activated and non-treated neutrophil membranes with the RP-1 mAb produced two bands of approximately 85kDa in M.W. However, the RP-1 antigen has not been biochemically characterized.

Host Species: Mouse
Clone: RP-1
Isotype: IgG2a κ
Species Reactivity: Rat
Immunogen: WKA/Hok rat peritoneal neutrophils

Flow Cytometry

Specifications

• Antigen: Granulocytes
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: WKA/Hok rat peritoneal neutrophils
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Rat
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: RP-1
• Conjugate: PE
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.