FoxP3 Mouse anti-Human, V450, Clone: 236A/E7, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 561182

Product Code. 15810839

Description

The 236A/E7 antibody reacts with the human FoxP3 (Forkhead box protein P3) transcription factor, a member of the forkhead or winged helix
family of transcription factors. The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+
CD25+ regulatory T cells and represents a specific marker for these cells. Flow cytometric analysis has shown that FoxP3 is expressed by the
majority of CD4+ CD25+ (high) T cells in peripheral blood while less than half of CD4+ CD25+ (intermediate) cell population are FoxP3
positive. Approximately 5-10% of peripheral CD4+ cells are CD4+ CD25+ T regulatory cells. T regulatory cells are thought to play a critical
role in the control of T cell mediated autoimmunity by suppressing the proliferation and cytokine production of other T cells. To support this
hypothesis, it has been found that Foxp3 is mutated in scurfy (sf) mice. Cumulative evidence suggests that the 236A/E7 antibody recognizes
epitopes from both isoforms of Exon 2 alternatively-spliced variants and is located in the region between Exon 2 and the Zn/LZ domains (aa
105-235). The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is
available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with
BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Host Species: Mouse
Clone: 236A/E7
Isotype: IgG1κ
Species Reactivity: Human
Immunogen: Human full-length FoxP3 Recombinant Protein

Flow Cytometry, Intracellular Staining

Specifications

• Antigen: FoxP3
• Classification: Monoclonal
• Conjugate: Violet Fluor 450 (V450)
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Human full-length FoxP3 Recombinant Protein
• Quantity: 100 Tests
• Research Discipline: Immunology
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: 236A/E7
• Description: Forkhead box protein P3; Scurfin; AIID; IPEX; JM2; DIETER; PIDX; XPID
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.