Invitrogen™ FOXP3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA146126

Product Code. 11548950

Description

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Suggested positive control: mouse CD4+CD25+ T cells. Epitope affinity purified.

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

Specifications

• Antigen: FOXP3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Foxp3
• Gene Alias: AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20371, 317382, 506053, 50943
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization (ISH), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: Q99JB6, Q9BZS1
• Gene Symbols: Foxp3
• Immunogen: A peptide corresponding to amino acids 43-100 of mouse FOXP3.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG