FoxP3 Mouse anti-Human, Alexa Fluor 647, Clone: 236A/E7, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 561184

Product Code. 15830839

Description

The 236A/E7 antibody reacts with the human FoxP3 (Forkhead box protein P3) transcription factor, a member of the forkhead or winged helix
family of transcription factors. The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+
CD25+ regulatory T cells and represents a specific marker for these cells. Flow cytometric analysis has shown that FoxP3 is expressed by the
majority of CD4+ CD25+ (high) T cells in peripheral blood while less than half of CD4+ CD25+ (intermediate) cell population are FoxP3
positive. Approximately 5-10% of peripheral CD4+ cells are CD4+ CD25+ T regulatory cells. T regulatory cells are thought to play a critical
role in the control of T cell mediated autoimmunity by suppressing the proliferation and cytokine production of other T cells. To support this
hypothesis, it has been found that Foxp3 is mutated in scurfy (sf) mice. Cumulative evidence suggests that the 236A/E7 antibody recognizes
epitopes from both isoforms of Exon 2 alternatively-spliced variants and is located in the region between Exon 2 and the Zn/LZ domains (aa
105-235). The antibody was conjugated to Alexa Fluor™ 647 under optimum conditions, and unreacted Alexa Fluor™ 647 was removed. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Host Species: Mouse
Clone: 236A/E7
Isotype: Mouse IgG1κ
Species Reactivity: Human
Immunogen: FoxP3 recombinant protein

Intracellular Staining

Specifications

• Antigen: FoxP3
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Human full-length FoxP3 Recombinant Protein
• Quantity: 100 Tests
• Research Discipline: Immunology
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: 236A/E7
• Description: Forkhead box protein P3; Scurfin; AIID; IPEX; JM2; DIETER; PIDX; XPID
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.