eNOS (pS633) Mouse anti-Human, Unlabeled, Clone: 37, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 612665

Product Code. 15836259

Description

Nitirc oxide synthase (NOS), a cell type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca²⁺; levels and enhance calmodulin binding. Neuronal NOS (nNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin. eNOS has a unique N-myristylation consensus sequence that may explain its membrane localization. Various protein kinases have been implicated in regulation of eNOS activity, including AMPK, PKA, PKB/Akt, PKC, and CaM Kinase II. During VEGF stimulation, eNOS is transiently phosphorylated at Ser-1177 by PKB/Akt and dephosphorylated at Thr-495. At later time points, VEGF stimulation leads to an increase in Thr-495 phosphorylation mediated by PKC and a decrease in Ser-1177 phosphorylation. In addition, Ser-633 amd Ser-1177 are phosphorylated by PKA and PKG in vitro. Thus, eNOS activity may be regulated through complex phosphorylation events mediated by multiple kinases at various phosphorylation sites.

Western Blotting

Specifications

• Antigen: eNOS (pS633)
• Classification: Monoclonal
• Concentration: 250μg/mL
• Description: Nitric Oxide Synthase
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

• Applications: Western Blot
• Clone: 37
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human eNOS (pS633) Peptide
• Quantity: 150 μg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1

For Research Use Only.