Invitrogen™ Endothelium Monoclonal Antibody (EN4)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA135359

Product Code. 11560812

Description

This antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.

The endothelium is the layer of thin specialized epithelium, comprising a simple squamous layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall (simple squamous epithelium). Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. These cells reduce friction of the flow of blood.

Specifications

• Antigen: Endothelium
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.1% BSA and 0.02% sodium azide
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Target Species: Human, Feline, Guinea Pig
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen)
• Clone: EN4
• Conjugate: Unconjugated
• Gene Alias: Endothelial cell marker; Endothelial cells; Endothelium Cell Antigen-1; Pan endothelial cells
• Immunogen: Proprietary Immunogen.
• Quantity: 1 mL
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid