E6AP Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA3843

Product Code. 11598841

Description

PA3-843 detects Angelman Syndrome-Associated protein (E6-AP) from human tissues. PA3-843 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ∽100 kDa protein representing E6-AP from HeLa cell lysate. IMPORTANT: E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots. PA3-843 antigen is bacterially expressed, full-length human E6-AP.

UBE3A (ubiquitin-protein ligase E3A, AKA E6AP) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. UBE3A also plays an important role in the regulation of the circadian clock. Defects in UBE3A are a cause of Angelman syndrome (AS).

Specifications

• Antigen: E6AP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UBE3A
• Gene Alias: 4732496B02; 5830462N02Rik; A130086L21Rik; ANCR; AS; CTCL tumor antigen se37-2; E6AP; E6-AP; E6-AP ubiquitin protein ligase; E6AP ubiquitin-protein ligase; EPVE6AP; FLJ26981; HECT-type ubiquitin transferase E3A; HPVE6A; human papilloma virus E6-associated protein; human papillomavirus E6-associated protein; mKIAA4216; oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; UBE3A; ubiquitin conjugating enzyme E3A; ubiquitin protein ligase E3A; ubiquitin-protein ligase E3A
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunoprecipitation, Western Blot
• Concentration: Conc. Not Determined
• Formulation: whole serum diluted in PBS with 0.05% sodium azide
• Gene Accession No.: Q05086
• Gene Symbols: UBE3A
• Immunogen: Full-length human E6-AP:GST fusion protein.
• Regulatory Status: RUO
• Gene ID (Entrez): 7337
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid