E2F-1 Mouse anti-Human, Unlabeled, Clone: KH95/E2F, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 554213

Product Code. 15827618

Description

The transcription factor E2F was originally characterized as a sequence-specific DNA-binding factor bound to the adenovirus E2A promoter. E2F-binding sites have now been identified in cellular promoters for genes involved in growth regulation, including c-myc, N-myc, Cdc2 and cyclin A. They have also been identified in promoters for genes whose products are required for DNA synthesis and replication, such as DNA polymerase a, thymidine kinase, thymidylate synthase, and dihydrofolate reductase. When bound to DNA, E2F has been found in complexes with key regulators of cell proliferation including the retinoblastoma protein (Rb) and the Rb related proteins, p107 and p130. Like a variety of other transcription factors, E2F binds to DNA as a heterodimer. The protein E2F-1 has E2F-like properties, it mediates E2F-dependent trans-activation and binds to underphosphorylated Rb. E2F dimerizes with DP-1, a protein which also has E2F-like properties. This dimerization leads to increased DNA binding activity and enhanced transcriptional activity. It is thought that E2F-1/DP association forms an active “E2F” transcription factor. E2F-1 migrates at a reduced molecular weight of ∽60kDa. Clone KH95/E2F (also referred to as KH95) recognizes an epitope between amino acids 342 and 386 of human E2F-1. It cross reacts with mouse and rat E2F-1. Full length, bacterially expressed, recombinant histidine-tagged human E2F-1 (amino acids 1-437) was purified and used as immunogen. Positive tissue culture supernatants were identified by immunoprecipitation of [35S]methionine-labeled, in vitro synthesized E2F-1 protein.

Host Species: Mouse
Clone: KH95/E2F
Isotype: IgG2a
Species Reactivity: Human
Formula Weight [Chemical]: 60kDa

Immunoprecipitation, Western Blotting

Specifications

• Antigen: E2F-1
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Human E2F-1 recombinant protein aa. 1-437
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human, Mouse, Rat
• Isotype: IgG2a

• Applications: Western Blot
• Clone: KH95/E2F
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.