DNMT1 (DNA Methyl Transferase-1) Mouse anti-Human, Unlabeled, Clone: 18, BD

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15856249	BD Biosciences 612618	50 μg
15866249	BD Biosciences 612619	150 μg

Description

DNA methylation of the 5′ position of the cytosine ring within CpG dinucleotides is essential for embryonic development. This methylation may affect transcriptional repression, formation of compact chromatin structures, X chromosome inactivation, and imprinting control. Three important DNA (Cytosine-5) methyltransferases (DNMTs) include DNMT1, DNMT3A, and DNMT3B. DNMT1 contains an N-terminal regulatory domain that may target it to replication foci, and a C-terminal catalytic domain that is homologous to bacterial cystosine 5-methylases. DNMT1 knockout mice have reduced DNA methylation, defects in imprinting, and derepression of endogenous retroviruses, and are embryonic lethal. DNMT1 is responsible for DNA methylations that occur after replication, while DNMT3A and DNMT3B act to establish new methylation patterns during embryogenesis. DNMT1 and DNMT3A are expressed in most fetal tissues, while DNMT3B is expressed only in fetal liver. All of these DNMTs are found in various adult tissues and show increased expression in some tumors and cancer cell lines. Thus, DNMT methylation of CpG dinucleotides is important for regulating DNA structure during development. This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or repoerted in the literature.

Immunofluorescence, Western Blotting

Specifications

• Antigen: DNMT1 (DNA Methyl Transferase-1)
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgG2b
• Purification Method: Affinity Purified
• Description: DNA Methyl Transferase-1

• Clone: 18
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human
• Immunogen: Human DNMT1 aa. 476-670
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.