DNase I Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA522017

Product Code. 12616213

Description

Recommended positive controls: Molt-4. Predicted reactivity: Dog (81%), Rabbit (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.

Specifications

• Antigen: DNase I
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DNASE1
• Gene Alias: AI788650; deoxyribonuclease; Deoxyribonuclease 1; deoxyribonuclease I; Deoxyribonuclease1; Deoxyribonuclease-1; DeoxyribonucleaseI; DNASE 1; DNase I; DNase I lysosomal; DNase I, lysosomal; Dnase1; DNaseI; DNL 1; DNL1; Dornase alfa; DRNI; epithelial lens deoxyribonuclease I; FLJ38093; human urine deoxyribonuclease I; pancreatic deoxyribonuclease I
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1773
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1.19 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P24855
• Gene Symbols: DNASE1
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 282 of DNase I (Uniprot ID#P24855)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG