Invitrogen™ POLI Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA529442

Product Code. 13493427

Description

Recommended positive controls: PC-3, HEK293. Predicted reactivity: Mouse (88%), Rat (85%), Chimpanzee (98%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Specifications

• Antigen: POLI
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Poli
• Gene Alias: DNA polymerase iota; eta2; Poli; polymerase (DNA directed) iota; polymerase (DNA directed), iota; polymerase (DNA) iota; polymerase (DNA-directed), iota; RAD 30B; RAD30; RAD30 homolog B; RAD30B; RAD3OB
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11201
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9UNA4
• Gene Symbols: Poli
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DNA polymerase iota. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG