DNA Polymerase beta Monoclonal Antibody, Clone: 61, Invitrogen™

DNA Polymerase beta Antibody (61), MA5-12086, from Invitrogen™. Species Reactivity: Human; Applications: Western Blot.

Overview
Brand: Invitrogen

Manufacturer Part Number: MA5-12086

DNA Polymerase beta (61) Mouse Monoclonal

UNSPSC: 12352203

Code: LE

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11334573

Quantity Price
1 £ 314.0 / 500µL
Estimated Shipment date
from Supplier 06-03-2017
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Description and Specification

Specification

Formulation 500μL purified antibody (0.2mg/mL) in PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
Gene ID (Entrez) 5423
Clone 61
Antigen DNA Polymerase beta
Storage Requirements Store at 4° C
Cross Reactivity Human
Immunogen DNA polymerase beta protein
Isotype IgG2a
Primary or Secondary Primary
Format Purified
Gene Accession No. P06746
Regulatory Status RUO
Host Species Mouse
Species Reactivity Human
Conjugate Unlabeled
Gene Alias DNA pol beta, DNA polymerase beta, DNA polymerase beta subunit
Gene Symbols POLB
Applications Western Blotting
Quantity 500μL
Monoclonal or Polyclonal Monoclonal

DNA polymerase beta comprises an amino-terminal 8-kDa domain and a carboxy-terminal 31-kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
Host Species: Mouse
Cross Reactivity: Human
Isotype: IgG2a
Clone: 61
Immunogen: DNA polymerase beta protein
Formulation: 500μL purified antibody (0.2mg/mL) in PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
Synonyms: DNA pol beta, DNA polymerase beta, DNA polymerase beta subunit
Uniprot Accession: P06746
Entrez GeneID: 5423
Gene Symbol: POLB
MA5-12086 targets DNA Polymerase beta in WB applications and shows reactivity with Human samples. The MA5-12086 immunogen is dNA polymerase beta protein.
References:
Test: WB, Dilution: 1:1000, Species: Hu, Reference: HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. (2014) Nat Commun, Vol: 5, , 5513, Fang Q,Inanc B,Schamus S,Wang XH,Wei L,Brown AR,Svilar D,Sugrue KF,Goellner EM,Zeng X,Yates NA,Lan L,Vens C,Sobol RW; Pubmed ID: 25423885