Disialoganglioside GD3 Mouse anti-Human, Unlabeled, Clone: MB3.6, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 554274

Product Code. 15857638

Description

Gangliosides are sialic-acid bearing glycolipids expressed on the surface of all mammalian cells, and are likely involved in mediating cell-substratum interactions. They are important target antigens for antibody mediated cytolysis of human melanoma and neuroblastoma cells. Human melanoma cells produce gangliosides GD2 and/or GD3 which are present in substratum-attached material, and may play a significant role in the melanoma metastatic phenotype. Ganglioside GD3 is a major surface marker on most human melanoma cells. MB3.6 has been used to localize GD3 in the plasma membrane and in focal adhesion plaques of human melanoma cells. Clone MB3.6 has also been shown to lyse GD3 positive human melanoma cells by both antibodydependent and complement-mediated cytotoxicity, and to inhibit the growth of human melanoma cells in athymic nude mice. Clone MB3.6 specifically reacts with human GD3 ganglioside. It does not cross-react with a variety of other gangliosides purified from melanoma or neuroblastoma cells. The FM9 human melanoma cell line was used as the immunogen.

Host Species: Mouse
Clone: MB3.6
Isotype: IgG3, κ
Species Reactivity: Human
Immunogen: FM9 human melamona cell line

Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry (Frozen)

Specifications

• Antigen: Disialoganglioside GD3
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: FM9 human melamona cell line
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG3 κ

• Applications: Flow Cytometry
• Clone: MB3.6
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.