CXCR4/CD184 Monoclonal Antibody, Clone: 12G5, Invitrogen™
CXCR4/CD184 Antibody (12G5), 35-8800, from Invitrogen™. Species Reactivity: Human; Applications: Flow Cytometry, Immunofluorescence, Western Blot
Brand: Invitrogen 358800
Code : 50
Additional Details : Weight : 0.50000kg
DescriptionHuman immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
Host Species: Mouse
Cross Reactivity: Human
Isotype: IgG2a, κ
Immunogen: SIV-infected SupT1 cells injected into Balb/c mice.
Formulation: 100μg purified antibody (0.5mg/mL) in PBS and 0.1% sodium azide; pH 7.4
Synonyms: D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, C-X-C chemokine receptor type 4
Uniprot Accession: P61073
Entrez GeneID: 7852
Gene Symbol: CXCR4
By Western blot, 35-8800 detects a band at ∽60kD, which likely represents an isoform or post-translationally modified form of CXCR4, many of which have been described in the literature.
Test: IHC (P), Dilution: 1:50, Species: Hu, Reference: Immunohistochemical analysis of cancer stem cell markers in pancreatic adenocarcinoma patients after neoadjuvant chemoradiotherapy. (2014) BMC Cancer, Vol: 14, , 687, Mizukami T,Kamachi H,Mitsuhashi T,Tsuruga Y,Hatanaka Y,Kamiyama T,Matsuno Y,Taketomi A; Pubmed ID: 25240521
|Store at -20°C|
|100μg of Protein A antibody in PBS with 0.1% sodium azide; pH 7.4.|
|SIV-infected SupT1 cells injected into Balb/c mice.|