Gibco™ Anti-Clumping Agent

Product Code.	Quantity	unitSize
11574536	20 mL	20mL
15234475	100 mL	100mL

Product Code. 11574536 Supplier Gibco™ Supplier No. 0010057AE

Description

Anti-Clumping Agent

GIBCO™ Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition.

Reduced cell clumping in suspension culture

Higher viable cell densities

Animal origin free (AOF) and chemically defined components

Convenient packaging with a larger size now available

Customization available to configure packaging and delivery

Simple and Gentle Cell Dissociation
Cell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping” state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly.

Reduced Clumping, Increased Viability, Improved Protein Expression
Reduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield.

For actual cell dissociation, we recommend our animal origin-free TrypLE™ products.

Use Anti-Clumping Agent after Transfection
Anti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection.

Animal Origin Free and Chemically Defined Components
Because Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch.

Convenient and Customizable Packaging
In addition to this 20 ml size, Anti-Clumping Agent is also available in a more economical and convenient 100 mL bottle. For even larger packaging, you can custom configure this product by clicking the 'Customize this Product' link under the 'Add to Cart' button above or by contacting us at CustomMedia@lifetech.com.

Additional Information and Support
Please review the Manuals and Protocols provided below for additional information on successful use of this product. Direct any unanswered questions to our Technical Services group.

For Research Use Only. Not for use in diagnostics procedures.

Specifications

• Content And Storage: Store at 2 to 8°C
• Cell Line: CHO, Insect Cells, 293 (HEK)
• Cell Type: Mammalian Cells
• Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
• Culture Type: Suspension Cell Culture
• Form: Liquid
• Product Type: Anti-clumping Reagent
• Format: Bottle(s)
• pH: 6 to 8
• Product Line: Gibco
• Quantity: 20 mL
• Shipping Condition: Room Temperature
• Source: Animal origin-free

Frequently Asked Questions (FAQs)

How much Anti-clumping agent should I use?

Use anti-clumping agent at a dilution of 1:250 to 1:1000 in culture medium. Test for impact on the cell culture and reduce concentration as the cell clumping is reduced.

Are there any recommendations for preventing or dispersing cell clumps in a suspension culture?

When cells meant to be grown in suspension are grown in static culture, they may form clumps. These clumps will severely limit transfection efficiency and protein expression. It is suggested that FreeStyle 293 cells in FreeStyle media and CHO-S cells in CD-CHO or CHO-SFM are grown in agitated suspension to reduce the appearance of clumps. However, if clumps do form, you can try the following protocol to select for cells that don't form clumps:

- Transfer cells into an appropriate size centrifuge tube that will hold the entire cell suspension.
- Allow cells to sit undisturbed for about 5 minutes. The time can vary depending on the specific cell line. Larger cell clumps will settle to the bottom of the tube.
- Collect cells from the upper portion of the tube to passage into a new flask.

My suspension cells are clumping together. What is going on?

There are a number of factors that can contribute to this:<

1) Presence of calcium and magnesium ions. Wash cells in a balanced salt solution without calcium and magnesium. Gently pipette cells to obtain a single cell suspension.

2) Mycoplasma contamination. Segregate culture and test for mycoplasma infection. Clean hood and incubator. If culture is contaminated, discard.

3) Cell lysis and release of DNA resulting from overdigestion with proteolytic enzymes. Treat cells with DNase I.

4) Certain suspension cell type try to aggregate when they are not under optimal agitation speed and optimization is required.

5) Certain suspension cell type try to aggregate in bioreactors and it is recommended to use Anti-Clumping Agent (Cat. No. 0010057AE).

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).