Cdk1/Cdc2 (pY15) Mouse, Unlabeled, Clone: 44, BD

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15886159	BD Biosciences 612306	50 μg
15896159	BD Biosciences 612307	150 μg

Description

Progression of the mammalian cell cycle is regulated by phosphorylation of many key proteins. Several classes of cyclins (A-E) act as regulatory subunits for cyclin-dependent kinases (cdks). Cdk1/Cdc2 (p34 [cdc2]) is the catalytic subunit of the maturation promoting factor (MPF), which includes the regulatory subunit cyclin B. During late S and G2 phase, cyclin B synthesis increases, allowing it to bind Cdc2. This begins the transition into M-phase of the mammalian cell cycle by initiating a series of phosphorylation and dephosphorylation events that lead to activation of the Cdc2/cyclin B complex. After binding to cyclin B, cdc2 is phosphorylated on Thr-14, by Myt1, and Tyr-15, by wee1 or mik1, yielding an inactive pre-MPF complex during G2 phase. Phosphorylation of cdc2 on Thr-161 is performed by a cdk7/cyclin H complex and is necessary for activation of the cdc2 complex. Dephosphorylation of Thr-14 and Tyr-15 by CDC25 occurs at the end of G2 phase and completes activation of the cdc2/Cyclin B complex and facilitates entry into mitosis. During mitosis, cyclin B is targeted for degradation and Cdc2 becomes inactive again.

Immunofluorescence, Western Blotting

Specifications

• Antigen: Cdk1/Cdc2 (pY15)
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgG1
• Purification Method: Affinity Purified
• Description: p34 [cdc2]

• Clone: 44
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat
• Immunogen: Phosphorylated Human Cdc2 (pY15) Peptide
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.