CD8a Mouse anti-Pig, PE, Clone: 76-2-11, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 559584

Product Code. 15819748

Description

The 76-2-11 (also known as clone PT8) antibody reacts with an epitope on the CD8α chain, a 35kDa antigen expressed on thymocytes, peripheral T lymphocytes, and NK cells. The CD8 molecule can exist as a 70kDa homodimer, composed of α chains, or heterodimer, composed of an α and β chain. Cells which express the CD8αα homodimer display dimmer staining with mAb 76-2-11 than CDαβ-expressing cells. The 76-2-11 mAb does not cross-react with human or bovine cells. Two peripheral CD8+ T-cell populations can be distinguished in the pig: CD8-bright CD4- CTL effectors/precursors and CD8-dull CD4+ T-helper lymphocytes. Pig NK cells express CD8 (dull staining), CD2, MHC class II, LFA-1, and asialo-GM1, but not CD3, CD4, CD5, or CD6. mAb 76-2-11 has been reported to partially inhibit in vitro cytotoxic activity of PBL to allogeneic leukocytes, but not NK-cell-mediated lysis, and to deplete CD8+ T cells in vivo. This clone was clustered as anti-CD8a at the First International Swine CD workshop.

Host Species: Mouse
Clone: 76-2-11
Isotype: IgG2a κ
Species Reactivity: Po
Immunogen: dd miniature swine thymocytes

Flow Cytometry

Specifications

• Antigen: CD8a
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: dd miniature swine thymocytes
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Pig
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: 76-2-11
• Conjugate: PE
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.