CD86 Rat anti-Mouse, V450, Clone: GL1, BD

Rat Monoclonal Antibody

Brand:  BD Biosciences 560450

Product Code. 15860209

Description

The GL1 antibody has been reported to react with the B7-2 (CD86) costimulatory molecule expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, thioglycollate-induced peritoneal macrophages, dendritic cells and astrocytes. CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T cell- and B cell-specific stimuli with a peak expression after 18-42 hours of culture. In contrast to most naive CD4+ T cells, memory CD4+ T cells express B7-2, both at the mRNA and protein level. CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role
in T cell-B cell costimulatory interactions. It has been shown to be involved in immunoglobulin class-switching and triggering of mouse NK cell-mediated cytotoxicity. CD80 (B7-1) is an alternate ligand for CD28 and CD152 (CTLA-4). GL1 antibody reportedly blocks MLR and stimulation of T cells by natural antigen-presenting cells. In addition, a mixture of anti-B7-1 and anti B7-2 (GL1) mAbs reportedly inhibits the in vitro interaction of CTLA-4 with its ligand and the in vivo priming of cytotoxic T lymphocytes. The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Host Species: Rat
Clone: GL1
Isotype: IgG2a κ
Species Reactivity: Mouse
Immunogen: Mouse (CBA/Ca) LPS-activated splenic B Cells

Flow Cytometry

Specifications

• Antigen: CD86
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: B7-2; Ly-58; Cd28l2; Early T-cell costimulatory molecule 1; ETC1; MB7; CLS1
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: GL1
• Conjugate: Violet Fluor 450 (V450)
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Mouse (CBA/Ca) LPS-activated splenic B Cells
• Quantity: 25 μg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2a κ

For Research Use Only.