CD62E Rat anti-Mouse, PE, Clone: 10E9.6, BD

Rat Monoclonal Antibody

Brand:  BD Biosciences 553751

Product Code. 15897368

Description

The 10E9.6 monoclonal antibody specifically binds to the 97-110 kDa cell surface glycoprotein E-selectin (CD62E), also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1), which is expressed on endotoxin- or cytokine-stimulated mouse endothelial cells. A suspension of TNF α stimulated mouse brain capillary endothelioma cells, from the cell line bEnd.3, was used as the immunogen. The epitope recognized by mAb 10E9.6 has been mapped to the first and/or second complement regulatory protein repeat domains of E-selectin. The 10E9.6 antibody has been reported to block binding of a monocyte cell line to E-selectin in vitro and to block neutrophil migration in BALB/c, but not C57BL/6 mice. It has no effect on leukocyte rolling in TNF α -treated mouse venules or on in vitro adhesion of myeloid cells to E-selectin. Studies have demonstrated that Cutaneous Lymphocyte Antigen (CLA), recognized by mAb HECA-452 (Cat. no. 555946), may be a ligand for CD62E.

Specifications

• Antigen: CD62E
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Sele; E-selectin; Elam; ELAM-1; LECAM2; LYAM2
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 10E9.6
• Conjugate: PE
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Mouse brain capillary endothelioma bEnd.3 (TNFα-stimulated) Cell Line
• Quantity: 0.2 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2a κ

For Research Use Only.