CD45 Mouse, Unlabeled, Clone: 69, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 610265

Product Code. 15865369

Description

CD45 (also known as leukocyte common antigen, GP180, or T200) is found on all hematopoietic cells except those of red cell origin. The N-terminal domain is a large glycosylated extracellular region of variable length (390-542 amino acids) derived by the alternative splicing of at least three exons (4, 5, or 6). This variation accounts for the different isoforms of CD45 (180-220 kDa). The remainder of the molecule includes a short transmembrane region followed by a large highly conserved cytoplasmic domain of 702 residues. The CD45 cytoplasmic domain possesses an intrinsic protein tyrosine phosphatase (PTPase) activity. This region contains two PTPase domains (domains I and II) which share 40% homology. Both domains are required for optimal PTPase activity and show significant homology with other receptor-like and cytoplasmic PTPases. CD45 plays a critical role in antigen receptor-induced responses of T and B cells. In B cells, CD45 can exist as part of a larger multisubunit complex composed of several tyrosine phosphorylated proteins, including the Src-family PTK, Lyn, or it can exist as the membrane immunoglobulin-associated MB-1/B29 heterodimer. In T cells, CD45 associates with and dephosphorylates both lck and fyn. In addition, CD45 can associate with membrane proteins involved in T cell activation, such as CD2, Thy-1, TCR, and CD26.

Specifications

• Antigen: CD45
• Classification: Monoclonal
• Concentration: 250μg/mL
• Description: Leukocyte Common Antigen; GP180; T200
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

• Applications: Western Blot
• Clone: 69
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Quantity: 50 μg
• Research Discipline: Immunology
• Target Species: Human, Mouse, Rat
• Isotype: IgG1

For Research Use Only.