missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. CD4 Mouse anti-Human, V500, Clone: RPA-T4, BD

CD4 Mouse anti-Human, V500, Clone: RPA-T4, BD

Product Code. 15820479
Change view
Click to view available options
Quantity:
100 Tests
25 Tests
Unit Size:
Each
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
15820479 25 Tests Each
12850490 100 Tests Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
This item is not returnable. View return policy
Product Code. 15820479 Supplier BD Biosciences Supplier No. 560769

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Mouse Monoclonal Antibody

The RPA-T4 clone reacts with CD4, a 59kDa single-chain transmembrane glycoprotein [receptor for human immunodeficiency virus (HIV)] present on T-helper/inducer cell populations. This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and reacts with approximately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral blood monocytes. RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation. The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser with an Ex max of 415 nm and Em Max at 500nm. BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel. For more information on BD Horizon V500, visit bdbiosciences.com/colors. When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls. Due to spectral differences between cells and beads in this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different V500 reagents (e.g. CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

Flow Cytometry

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD4
Applications Flow Cytometry
Classification Monoclonal
Clone RPA-T4
Conjugate Violet Fluor 500 (V500)
Formulation Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.
Host Species Mouse
Purification Method Affinity Purified
Quantity 25 Tests
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
Show More Show Less

For Research Use Only.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.