CD34 Monoclonal Antibody (581), PE, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA516526

Product Code. 15225819

Description

For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. The recommended shelf-life for this product is 6 months. Mouse anti Human CD34 antibody, clone 581 recognizes the human CD34 cell surface antigen also known as Hematopoietic progenitor cell antigen CD34.

CD34 is a highly glycosylated, monomeric, 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation, and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

Specifications

• Antigen: CD34
• Classification: Monoclonal
• Conjugate: PE
• Gene Accession No.: P28906
• Gene Symbols: CD34
• Immunogen: The details of the immunogen for this antibody are not currently available.
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human, Non-human Primate
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: 581
• Gene: CD34
• Gene Alias: Mucosialin, RP11-328D5.2, CD34 antigen, hematopoietic progenitor cell antigen CD34
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 947
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid