CD33 Mouse anti-Human, Brilliant Violet 421, Clone: WM53, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 562854

Product Code. 15862299

Description

The WM53 monoclonal antibody specifically binds to human CD33. CD33 is a 67 kDa type I transmembrane glycoprotein. It is expressed on monocytes, activated T cells, myeloid progenitors, mast cells and weakly on polymorphonuclear cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes, and hematopoietic stem cells. Reports indicate that this glycoprotein can function as a sialic acid-dependent cell adhesion molecule and this function can be modulated by endogenous sialoglycoconjugates when CD33 is expressed on the membrane.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

Specifications

• Antigen: CD33
• Classification: Monoclonal
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Acute Myeloid Leukemia Blasts
• Quantity: 50 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: WM53
• Description: Siglec-3; SIGLEC3; Sialic acid-binding Ig-like lectin 3; p67; gp67; My9
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ