CD3 Mouse anti-Rat, PE, Clone: 1F4, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 550353

Product Code. 15846248

Description

The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD3
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: F344 rat splenic T cells stimulated with PMA plus A23187
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Rat
• Isotype: IgM κ

• Applications: Flow Cytometry
• Clone: 1F4
• Conjugate: PE
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.