CD25 Mouse anti-Human, BB515, Clone: 2A3, BD

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15833669	BD Biosciences 564468	25 Tests
15823669	BD Biosciences 564467	100 Tests

Description

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα). CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γchain or γc) to f orm the high-affinity signal-transducing IL-2R complex. CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. CD25 can also be expressed by activated B cells and macrophages. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes.

The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

• Antigen: CD25
• Applications: Flow Cytometry
• Conjugate: Brilliant Blue 515 (BB515)
• Regulatory Status: RUO
• Immunogen: Human Phytohemagglutinin-activated T Cells
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: 2A3
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55

For Research Use Only.