CD212 Mouse anti-Mouse, Biotin, Clone: 114, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 551973

Product Code. 15836748

Description

The 114 antibody reacts with mouse CD212 (the β1 subunit of IL-12Rβ1), originally termed IL-12Rβ, of the mouse IL-12 receptor complex. The IL-12Rβ1 subunit associates with a β2 subunit to form a heterodimeric IL-12 receptor complex. Each one of the IL-12R subunits exhibits low affinity for IL-12, but in combination, they bind IL-12 with high affinity. The IL-12Rβ1 subunit interacts primarily with IL-12 p40 whereas the IL-12Rββ1 has more recently been described to bind IL-23, a heterodimer formed of the p40 subunit from IL-12, and p19. The cytoplasmic regions of the β1 and β2 subunits contain the box1 and box2 motifs found in other cytokine receptors such as gp130, LIFR and G-CSFR. IL-12Rβ1 are primarily expressed by activated T cells and NK cells. Experiments with IL-12Rβ1 deficient mice have shown that IL-12Rβ1 is necessary for mouse T and NK cell responsiveness to IL-12 p75. The 114 antibody was generated by immunizing IL-12Rβ1 deficient mice of (129 x BALB/c)F1 background with mouse Ba/F3 cells that were stably transfected with IL-12Rβ1

Specifications

• Antigen: CD212
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: IL-12 Receptor β1 chain
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 114
• Conjugate: Biotin
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: IL-12Rβ1 Transfected Cell Line
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2a κ

For Research Use Only.